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Objective To investigate the effect of potassium channel blocker tetraethtylamine (TEA) on the proliferation and apoptosis of human laryngeal carcinoma cells (Hep-2) through the PI3K/Akt signaling pathway.Methods TEA acted on HEp-2.The methyl thiazolyl blue (MTT) method was used to determine the Hep-2 cell activity and the proliferation inhibition rate of TEA on Hep-2 was calculated;the Hoechest33258 staining was used to detect cell apoptosis,and the Hep-2 apoptosis rate was detected by flow cytometry (FCM) assay.Results After inoculation by 5,10,20 mmol/L TEA,the Hep-2 cell proliferation inhibition rate reached 12.573%,31.385% and 56.132%,respectively.After 96 h Hep-2 cell action,the cellular apoptosis rates were (41.64±2.67)%,(58.76±4.32)% and (72.65±6.54)% respectively,the inhibition rate and apoptosis rate in the TEA treating group were significantly higher than those in the non-TEA treating group,the differences were statistically significant (P<0.05).Conclusion TEA can inhibit the proliferation and in vivo growth of human laryngeal carcinoma cells and promotes the apoptosis of laryngeal cancer cells.
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OBJECTIVE@#To investigate the myeloid differentiation factor 88 (MyD88) expression in laryngeal carcinoma and its clinical significance.@*METHOD@#Fifty-one patients with laryngeal carcinoma were collected, and all patients were confirmed by pathological diagnosis results. The expression of MyD88 protein was detected by immunohistochemical method in laryngeal cancer and its adjacent tissues to investigate the correlation among MyD88 expression, clinicopathological characteristics and prognosis of patients.@*RESULT@#The positive expression rate of MyD88 in laryngeal cancer tissues was 68.6%, significantly higher than that in normal tissues adjacent to carcinoma of which positive expression rate was 11.8%; MyD88 positive rate had nothing to do with laryngeal cancer patients age, sex, differentiation and tumour location (all P > 0.05), but correlated with clinical stage (P < 0.01) and lymph node metastasis (P < 0.05). In addition, the study also found that the expression of MyD88 quantity was inversely proportional with the five-year survival rate. The survival rate of patients with higher expression of MyD88 was significantly lower than that of patients with lower expression (P < 0.05).@*CONCLUSION@#MyD88 may be an important participant in the pathogenesis of laryngeal carcinoma, MyD88 targeted therapy may improve the prognosis of patients with laryngeal cancer.
Subject(s)
Humans , Laryngeal Neoplasms , Metabolism , Pathology , Lymphatic Metastasis , Myeloid Differentiation Factor 88 , Metabolism , Prognosis , Survival RateABSTRACT
OBJECTIVE@#To investigate the expressions of RNA-dependent adenosine deaminase 1(ADAR1) mRNA in larynx carcinoma tissues, and to discuss its value in the development of larynx carcinoma.@*METHOD@#The expression of ADAR1 mRNA in 51 larynx carcinoma and peri-carcinoma tissues were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).@*RESULT@#ADAR1 mRNA was expressed broadly; the relative intensities of its expression in larynx carcinoma, larynx peri-carcinoma samples and larynx non-carcinoma tissue samples were respectively 2.963 +/- 0.912, 0.791 +/- 0.197 and 0.910 +/- 0.311. There were remarkable difference between larynx carcinoma and larynx peri-carcinoma, larynx carcinoma and non-carcinoma tissues.@*CONCLUSION@#ADAR1 mRNA is expressed broadly in larynx carcinoma and may be play an important role in the development of larynx carcinoma.
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Adenosine Deaminase , Genetics , Metabolism , Laryngeal Neoplasms , Genetics , Metabolism , Pathology , RNA, Messenger , Genetics , RNA-Binding ProteinsABSTRACT
OBJECTIVE To study the effect of blocking chloride channel on the proliferation and apoptosis of Hep-2 cell line.METHODS Hep-2 cell line was used as a target,The effect of chloride channel blocker(NPPB)on the proliferation of Hep-2 cell line was evaluated by MTT assay.Flow cytometry was performed to measure the apoptosis index of selected Hep-2 cell clones.RESULTS Inhibition of the proliferation of Hep-2 cells depended on the dose of chloride channel blocker(NPPB).The apoptosis index of Hep-2 cells was remarkably different before and after its chloride channel was blocked. CONCLUSION Normal expression and function of chloride channel is essential for the maintenance of proper cell growth.Our results suggest that blocking the chloride channel could inhibit the proliferation and induce apoptosis of Hep-2 cell line.
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OBJECTIVE To study the relationship between potassium channel and ADAR1 in Hep-2 cell line. METHODS The potassium current was recorded by the whole-cell recording technique of perforated membrane clamp. Using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) , the expressions of ADAR1 mRNA in the Hep-2cell line were detected. RESULTS The similar membrane current was observed when cells were held at -40 mV and test potentials ranged from -80 mV to +80 mV. The current exhibited properties of voltage-dependent, outward rectification. It exhibited complete activation after alatency of 25ms and no or little inactivation over the 800ms voltage pulse. It could be blocked by potassium channel blockers TEA. The relative intensities of ADAR1 mRNA of t he Hep-2 cell line were different before and after its potassium channel was blocked. CONCLUSION Delayed rectifier potassium channel exist in human laryngeal carcinoma cell line Hep-2.The potassium channel is voltage-dependent. There is a correlation between the potassium channel and ADAR1 mRNA in Hep-2 cell line.